Affiliation:
1. Biomolecular Sciences Division, King's College London, Campden Hill, London W8 7AH, UK
Abstract
Abstract
A new method for the preparation of liposomes is described that avoids the use of pharmaceutically unacceptable solvents and energy-expensive procedures such as sonication. The method is based on the initial formation of a proliposome mixture containing lipid, ethanol and water, which is converted to lipsomes by a simple dilution step. Measurements using 6-carboxyfluorescein as a marker indicate that water-soluble drugs can be trapped with extremely high efficiency (65–80% depending on lipid composition). The structural organization of the proliposome mixture and the final liposomes were characterized using electron microscopy and 31P-NMR.
Publisher
Oxford University Press (OUP)
Subject
Pharmaceutical Science,Pharmacology
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