Competitive Protein-binding Radioassay of Thiamine in Simple Solutions and in Multivitamin Pharmaceuticals-Reference-Cited by-同舟云学术

Competitive Protein-binding Radioassay of Thiamine in Simple Solutions and in Multivitamin Pharmaceuticals

Published:1991-05 Issue:5 Volume:43 Page:303-306
ISSN:2042-7158
Container-title:Journal of Pharmacy and Pharmacology
language:en
Short-container-title:

Author:

Kozik Andrzej¹,Wróabel Radoslawa¹,Turyna Izabela¹

Affiliation:

1. Jagiellonian University, Institute of Molecular Biology, Al. Mickiewicza 3, 31-120 Kraków, Poland

Abstract

Abstract A competitive inhibition radioassay of thiamine is described using a gel obtained by coupling a buckwheat-seed thiamine-binding protein to CNBr-activated Sepharose. The sample to be analysed is incubated with gel suspension and [14C]thiamine and after centrifugation the radioactivity of the supernatant is measured. The method is simple and specific, and applicable over a thiamine concentration range 0.5-5 μM with a coefficient of variation typically below 5%. The gel is reusable and stable for several months. Applicability of the method for direct determination of thiamine in multivitamin pharmaceuticals is demonstrated.

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

Link

http://academic.oup.com/jpp/article-pdf/43/5/303/37241257/j.2042-7158.1991.tb06694.x.pdf

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