Affiliation:
1. Pharmacology Group, School of Pharmacy, Bath University of Technology, Bath BA2 7AY, UK
Abstract
Abstract
The effect of anti-inflammatory steroids on lysosomal enzyme release has been investigated. Most of the steroids stabilized lysosomes at pharmacological concentrations (10−4–10−6M) but lysed them at higher concentrations. Etiocholanolone, a steroid pyrogenic in man, had no stabilizing effect. The concentration of steroid would therefore seem critical in determining its subcellular action. Experiments with albumin suggest that anti-inflammatory steroids (at 5 × 10−4M) have little effect in aiding its thermal denaturation whereas other steroids greatly increase denaturation. Increasing concentrations of cortisol and prednisolone however caused greater denaturation of albumin. Although the correlation between albumin solutions and lysosomal membrane proteins is tenuous it is suggested that the lytic effect of anti-inflammatory steroids could be due to protein denaturation. Their stabilizing effect, however, probably involves steroidlipid interactions.
Publisher
Oxford University Press (OUP)
Subject
Pharmaceutical Science,Pharmacology
Cited by
52 articles.
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