An HPLC assay procedure of sensitivity and stability for measurement of acetylcholine and choline in neuronal tissue-Reference-Cited by-同舟云学术

An HPLC assay procedure of sensitivity and stability for measurement of acetylcholine and choline in neuronal tissue

Published:1987-09 Issue:9 Volume:39 Page:727-731
ISSN:2042-7158
Container-title:Journal of Pharmacy and Pharmacology
language:en
Short-container-title:

Author:

Barnes N M¹,Costall B¹²,Fell A F¹,Naylor R J²

Affiliation:

1. Pharmaceutical Analysis Research Unit, Postgraduate School of Studies in Pharmaceutical Chemistry

2. Postgraduate School of Studies in Pharmacology, University of Bradford, Bradford, West Yorkshire BD7 1DP, UK

Abstract

Abstract A method is presented for the sensitive and specific determination of acetylcholine and choline in neuronal tissue. The method is based on the separation of acetylcholine and choline by reversed-phase HPLC, passing the eluent into a post-column reactor containing choline oxidase and acetylcholinesterase covalently bound to vinyl sulphone bonded onto a hydroxyethyl methacrylate support, and electrochemical detection of the hydrogen peroxide formed. The limit of detection of the procedure is 1 pmol for acetylcholine and 500 fmol for choline. The excellent baseline stability of the method ensures the rapid and reliable processing of a large number of samples.

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

Link

http://academic.oup.com/jpp/article-pdf/39/9/727/37545425/j.2042-7158.1987.tb06977.x.pdf

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