A highly sensitive HPLC method for the assay of propantheline used to measure its uptake by rat intestinal brush border membrane vesicles-Reference-Cited by-同舟云学术

A highly sensitive HPLC method for the assay of propantheline used to measure its uptake by rat intestinal brush border membrane vesicles

Published:1987-01 Issue:1 Volume:39 Page:9-12
ISSN:2042-7158
Container-title:Journal of Pharmacy and Pharmacology
language:en
Short-container-title:

Author:

Saitoh Hiroshi¹,Kobayashi Yukari¹,Miyazaki Katsumi¹,Arita Takaichi¹

Affiliation:

1. Department of Pharmacy, Hokkaido University Hospital, School of Medicine, Hokkaido University, Kita-14-jo, Nishi-5-chome, Kita-ku, Sapporo 060, Japan

Abstract

Abstract A simple, sensitive, high-performance liquid chromatographic method for propantheline has been developed. Propantheline was quantitatively hydrolysed into xanthene-9-carboxylic acid in neutral or alkaline medium and the hydrolysate assayed by reversed phase high-performance liquid chromatography. This method measured to 2 pmol per injection and was used to investigate the uptake of the drug by rat intestinal brush border membrane vesicles. Propantheline was highly bound to the membrane and this binding was inhibited to varying extents by several quaternary ammonium compounds. Mepenzolate and methylbenactyzium inhibited it significantly, and neostigmine, butylscopolamine and N-methylnicotinamide inhibited it moderately. Choline, acetylcholine and thiamine had no effect.

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

Link

http://academic.oup.com/jpp/article-pdf/39/1/9/37541504/j.2042-7158.1987.tb07153.x.pdf

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4. The absorption and distribution of isopropamide iodide in the rat

5. Enhancement of Intestinal Absorption of a Quaternary Ammonium Compound by Salicylate and Trichloroacetate

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