Alternative therapeutic advantages of catfish bile on atopic dermatitis: protection of T cell-mediated skin disease via antioxidant activities

Author:

Lee Do Ik1,Huh Yoon Joo1,Hwang Kwang Woo1,Choi YoungWook1,Choi Jae-Seok2,Han Sam-Yong3,Gyoung Young-Soo3,Joo Seong Soo4

Affiliation:

1. College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea

2. Institute of Environmental Research, Kangwon National University, Gangwon, Republic of Korea

3. Department of Chemistry, College of Natural Science, Gangneung-Wonju National University, Gangwon, Republic of Korea

4. Department of Marine Molecular Biotechnology, College of Life Science, Gangneung-Wonju National University, Gangwon, Republic of Korea

Abstract

Abstract Objectives In the present study, we aimed to examine the anti-atopic properties of bile from the cat fish, Silurus asotus, to determine its possible use as a pharmaceutical product. Methods The anti-atopic activities of cat fish bile were examined in a non-cell antioxidant, in-vitro assay (splenocytes and mast cells) and a 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis-like mouse model. Results The results of these experiments revealed that Silurus asotus bile (SAB) scavenges radicals and protects proteins from superoxide attacks, suggesting that SAB suppresses the T helper (Th) type 2-skewed immune response. Th1/Th2 mRNA cytokines (interleukin (IL)-2, interferon (IFN)-γ and IL-4) from mouse splenocytes were effectively inhibited, and the release of β-hexosaminidase in RBL-2H3 mast cells was significantly suppressed by SAB. These results were supported by screening the Th1/Th2 cytokine mRNAs (IL-2, IFN-γ and IL-4) from lymph nodes in DNCB-treated mice. More dramatic results were observed in the histological changes at higher SAB concentrations (5%) compared to the therapeutic control, visualized using hematoxylin–eosin (H&E) staining. Conclusions The results presented in this study suggest that SAB may provide functional advantages with regard to treating atopic dermatitis because of its antioxidant and immune-suppressive effects.

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

Reference22 articles.

1. Allergy and the skin;Incovaria;Clin Exp Immunol,2003

2. Keratinocytes act as a source of reactive oxygen species by transferring hydrogen peroxide to melanocytes;Pelle;J Invest Dermatol,2005

3. The role of redox regulation in the normal physiology and inflammatory disease of skin;Kornina;Front Biosci,2009

4. Antioxidant activity, lipid peroxidation and skin diseases;Briganti;J Eur Acad Dermatol,2003

5. Desferrioxamine modulates chemically induced T helper 2-mediated autoimmunity in the rat;Wu;Clin Exp Immunol,2004

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