Gastric blood flow responses to autonomic nerve stimulation and related pharmacological studies in rats

Abstract

Abstract The effects of autonomic nerve stimulation on rat gastric blood flow (GBF) were studied using a cross thermocouple method. Stimulation of the periarterial nerve bundles along the left gastric artery produced a decrease in GBF which was antagonized by phenoxybenzamine (0·05 mg kg−1 i.v.), but not by propranolol (1 mg kg−1 i.v.). Stimulation of the vagus nerves elicited an increase in GBF, within a latency of 20 s, which was not apparently affected by atropine (0·15 and 1·5 mg kg−1 i.v.) but was completely blocked by hexamethonium (10 mg kg−1 i.v.). The GBF increase due to acetylcholine (0·25 μg rat−1 i.a.) was markedly blocked by atropine (0·15 mg kg−1 i.v.). Vagal stimulation also produced both the cholinergic excitation and non-cholinergic inhibition of gastric motility. The vagally induced GBF increase was little affected by any pretreatment with phentolamine, propranolol, indomethacin or aprotinin. These results suggest that sympathetic nerve stimulation decreases GBF through α-adrenoceptors and parasympathetic nerve stimulation increases GBF through a non-cholinergic mechanism in rats and that the GBF increase may result from a primary dilator effect of vagal stimulation on the blood vessels because of the immediate initiation of the response.