Behavioural Pharmacological Characteristics of Honokiol, an Anxiolytic Agent Present in Extracts of Magnolia Bark, Evaluated by an Elevated Plus-maze Test in Mice

Abstract

Abstract Honokiol, a neolignane derivative of Magnolia bark, has central depressant action and, at much lower doses, anxiolytic activity. We have investigated the characteristics of the behavioural effects of honokiol by means of an elevated plus-maze test. In the plus-maze test a single oral dose of 20 mg kg−1 honokiol significantly prolonged the time spent in the open arms of the maze, suggesting anxiolytic effect. Moreover, when honokiol was administered daily for seven days and the plus-maze test was conducted 3 or 24 h after the last administration, significant prolongation of the time in the open arms was manifested even for doses of 0.2 mg kg−1. The maximum effect was observed for doses of 0.5 mg kg−1. Honokiol at any dose in both single and repeated administration schedules caused neither change in motor activity nor disruption of traction performance. Orally administered diazepam, 0.5–2 mg kg−1, caused dose-dependent prolongation of the time spent in the open arms of the maze with a significant increase in motor activity at 1 mg kg−1, and dose-dependent disruption of traction performance. The changes in the plus-maze performance after treatment for seven days with 0.2 mg kg−1 honokiol and after a single treatment with 1 mg kg−1 diazepam were almost equivalent. The effect of honokiol (0.2 mg kg−1, treatment for seven days) was inhibited by subcutaneous flumazenil (0.3 mg kg−1) and (+)-bicuculline (0.1 mg kg−1) and by intraperitoneal CCK-4 (50 μg kg−1) and caffeine (30 mg kg−1). The anxiolytic effect of diazepam (1 mg kg−1) was also inhibited by flumazenil and bicuculline. However, the combined administration of diazepam with caffeine enhanced the effect, and diazepam completely reversed the effect of CCK-4. These results suggest that, in contrast with diazepam, honokiol selectively induces an anxiolytic effect with less liability of eliciting motor dysfunction and sedation or dis-inhibition. The combined effects of the drug also revealed that the mechanism of anxiolytic effect of honokiol is partially different from that of diazepam.