LncRNA CARMN facilitates odontogenic differentiation of dental pulp cells by impairing EZH2

Author:

Li Hongyu123,Huo Sibei123,He Xinyu123,Guo Daimo123,Liu Yingling123,Zheng Liwei124,Zhou Xin124ORCID

Affiliation:

1. State Key laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University Chengdu China

2. National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University Chengdu China

3. West China School of Stomatology Sichuan University Chengdu China

4. Department of Pediatric Dentistry, West China Hospital of Stomatology Sichuan University Chengdu China

Abstract

AbstractObjectiveThis study aimed to reveal the potential role of CARMN in odontogenic differentiation of dental pulp cells (DPCs).MethodsLaser capture microdissection was used to detect Carmn in DPCs and odontoblasts in P0 mice. After manipulating CARMN expression in odontogenic differentiation induced hDPCs, the state of odontogenic differentiation was evaluated by ALP staining, ARS, and related marker expression in qRT‐PCR and western blotting. The subcutaneous transplantation of HA/β‐TCP loaded with hDPCs was performed to verify CARMN's role in promoting odontogenic differentiation in vivo. RNAplex and RIP were employed to reveal potential mechanism of CARMN in hDPCs.ResultsCARMN expressed more abundantly in odontoblasts than DPCs in P0 mice. CARMN expression boosted during in vitro odontogenic differentiation of hDPCs. CARMN overexpression enhanced odontogenic differentiation of hDPCs in vitro, while inhibition impaired the process. CARMN overexpression in HA/β‐TCP composites promoted more mineralized nodule formation in vivo. CARMN knockdown led to soared EZH2, while CARMN overexpression brought about EZH2 inhibition. CARMN functioned via direct interaction with EZH2.ConclusionsThe results uncovered CARMN as a modulator during the odontogenic differentiation of DPCs. CARMN promoted odontogenic differentiation of DPCs by impairing EZH2.

Funder

National Natural Science Foundation of China

Publisher

Wiley

Subject

General Dentistry,Otorhinolaryngology

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