Periodontitis‐resistant and ‐susceptible matriline regulation of gingival transcriptome in nonhuman primates

Abstract

AbstractObjectiveThis report identified if gingival gene expression transcriptomes demonstrated unique profiles that discriminated periodontitis‐susceptible (PDS) and periodontitis‐resistant (PDR) animals in health and disease.BackgroundNonhuman primates generally organize their social groups based upon matriline origin. We have used a multi‐generational colony of rhesus macaques to identify matrilines presenting with significant differences in periodontitis (e.g., earlier age onset, greater prevalence, and severity).MethodsAnimals from 12 to 23 years of age (n = 17; 8 – PDR, 9 – PDS) were entered into a ligature‐induced periodontitis trial. Gingival biopsies were taken at baseline and 0.5, 1, 3, and 5 months post‐ligation, and microarray analysis was used to quantify gene expression in samples at each time point.ResultsOver 1000 genes showed significant (p < .01) differences in the PDR versus PDS animals at baseline. The frequency of differences generally decreased during the disease process, and increased with resolution (i.e., 5 months). A nearly 2:1 ratio of elevated gene levels was noted in baseline PDR samples that included up‐regulated MMPs, Fc receptors, chemokines, interleukins, and innate immune receptors, and down‐regulated genes particularly related to epithelial biology. Most dramatically, there was a skewed differential expression of adaptive immune response genes in the PDR and epithelial cell structure/function genes in PDS samples.ConclusionsThe results demonstrate substantive differences in gingival tissue response capacity/programming in PDR and PDS samples that may contribute to the differences in clinical outcomes related to the heritability of disease risk through matrilines.