Mechanism of trophoblast cell‐derived microparticles mediated immunocontraceptive response

Abstract

AbstractProblemImmunomodulation profoundly affects the process of human implantation. Trophoblast cell‐derived microparticles (Tr‐MPs) may activate specific T cells to attack trophoblast cells, thus potentially acting as an immunocontraceptive vaccine. The safety and persistence of Tr‐MP vaccine are needed to address.Method of studyFlow cytometry and confocal fluorescent microscopy were conducted to detect cellular absorptivity and localization of Tr‐MPs in bone marrow‐derived dendritic cells (BMDCs). The phenotype and cytokine secretion of BMDC and T cells were performed by flow cytometry and enzyme‐linked immuno sorbent assay (ELISA). The constructed vaccine female moused model were used to observe the infertile effect and safety of Tr‐MPs.ResultsAs compared with non‐irradiation exposure groups, the number of MPs released by trophoblast cells in ultraviolet immunized groups significantly increased. The phagocytosis of Tr‐MPs led to the maturation of dendritic cells (DCs), which, in turn, activate T cells. Then cytotoxic T cells attacking trophoblast cells. In mouse model, female mice were infertile after receiving Tr‐MPs, and the effect of contraception is transient and safety.ConclusionUsing Tr‐MPs to initiate an adaptive immune response against alloantigens in trophoblast cells. Tr‐MPs may be a new candidate for the development of contraceptive vaccines due to its effectiveness, safety, and reversibility.