Immunohistochemical expression of beta‐catenin, BMP4 and TGF‐beta in odontomas

Author:

de França Glória Maria1ORCID,Pires Hévila de Figueiredo1,da Silva Weslay Rodrigues2,de Morais Éverton Freitas1,Freitas Roseana de Almeida1,de Souza Lélia Batista1,Galvão Hébel Cavalcanti1

Affiliation:

1. Postgraduate Program in Dental Sciences, Area of Concentration in Stomatology and Oral Pathology Federal University of Rio Grande do Norte Natal Brazil

2. School of Dentistry, Postgraduate Program in Dentistry University of Pernambuco (UPE) Recife Pernambuco Brazil

Abstract

AbstractChanges in the expression of nuclear β‐catenin are responsible for tumorigenesis. Beta‐catenin acts synergistically with the TGF‐β/BMPs pathway. This interaction leads to greater dentin deposition and may explain the differences between distinct tooth morphologies and hamartomas. The aim of this study was to investigate the role of β‐catenin, BMP4 and TGF‐β in the development of odontomas. This cross‐sectional, retrospective, immunohistochemical study evaluated 30 compound odontomas, 30 complex odontomas and 17 tooth germs. The results showed that BMP4 and TGF‐β were more immunoexpressed in the ectomesenchyme of complex odontomas (median = 33.7, p < 0.001; median = 76.4, p = 0.002, respectively). Higher immunoexpression of BMP4 and TGF‐β was also observed in the epithelium of tooth germs (median = 2.0, p < 0.001; median = 120.3, p < 0.001, respectively). TGF‐β and BMP4 showed a positive and significant correlation (p < 0.001). Both TGF‐β and BMP4 were positively correlated with nuclear β‐catenin in ectomesenchyme (p = 0.047 and p = 0.023, respectively). Developing teeth exhibited higher concentrations of the proteins studied in odontogenic epithelium, especially during the bud and cap stages. Higher immunoexpression in odontomas occurred mainly in the ectomesenchyme. We therefore suggest that changes in the ectomesenchyme can lead to the development of odontomas.

Publisher

Wiley

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