Affiliation:
1. Department of Periodontology Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai China
2. Shanghai Key Laboratory of Sleep Disordered Breathing, Otolaryngology Institute of Shanghai JiaoTong University, Department of Otolaryngology‐Head and Neck Surgery Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai China
Abstract
AbstractAimTo investigate the specific role of arrestin beta‐2 (ARRB2) in the progression of periodontitis and the underlying mechanisms.Materials and MethodsSingle‐cell RNA sequencing data were used to analyse gene expression in periodontal tissues from healthy controls and patients with periodontitis. Real‐time quantitative polymerase chain reaction, Western blotting and immunohistochemical staining were performed to detect the expression of ARRB2. Furthermore, a ligature‐induced periodontitis model was created. Using radiographic and histological methods, RNA sequencing and luciferase assay, the role of ARRB2 in periodontitis and the underlying mechanisms were explored. Finally, the therapeutic effect of melatonin, an inhibitor of activating transcription factor 6 (ATF6), on periodontitis in mice was assessed in both in vivo and in vitro experiments.ResultsARRB2 expression was up‐regulated in inflammatory periodontal tissue. In the ligature‐induced mouse model, Arrb2 knockout exacerbated alveolar bone loss (ABL) and extracellular matrix (ECM) degradation. ARRB2 exerted a negative regulatory effect on ATF6, an essential targeted gene. Melatonin ameliorated ABL and an imbalance in ECM remodelling in Arrb2‐deficient periodontitis mice.ConclusionsARRB2 mediates ECM remodelling via inhibition of the ATF6 signalling pathway, which ultimately exerts a protective effect on periodontal tissues.
Funder
National Natural Science Foundation of China