Affiliation:
1. Research Laboratory KinoPharma Inc. Kyoto Japan
2. Department of Microbiology and Immunology Hamamatsu University School of Medicine Hamamatsu Shizuoka Japan
Abstract
AbstractBK polyomavirus (BKPyV) was the first human polyomavirus to be isolated from an immunosuppressed kidney transplant recipient in 1971. BKPyV reactivation causes BKPyV‐associated nephropathy and hemorrhagic cystitis. However, the mechanisms underlying BKPyV replication remain unclear. In the present study, we performed the long‐term cultivation of COS‐7 cells transfected with archetype KOM‐5 DNA, which were designated as COS‐BK cells. BKPyV derived from COS‐BK cells was characterized by analyzing the amount of the virus based on hemagglutination, viral replication, and the production of viral protein 1 (VP1). Immunostaining showed that VP1‐positive cells accounted for a small percentage of COS‐BK cells. The nucleotide sequences encompassing the origin of the DNA replication of BKPyV derived from COS‐BK cells were generated from KOM‐5 by the deletion of an 8‐bp sequence, which did not involve T antigen binding sites. BKPyV replicated most efficiently in COS‐BK cells in DMEM containing 2% fetal bovine serum. These results indicate that COS‐BK cells are a suitable culture system for studying the persistent infection of archetype BKPyV.