Affiliation:
1. Department of Virology 3 National Institute of Infectious Diseases Tokyo Japan
2. Center for Influenza and Respiratory Virus Research National Institute of Infectious Diseases Tokyo Japan
Abstract
AbstractImmunological testing to detect neutralizing antibodies (NAbs) is important in measles (MV) infection control. Currently, the plaque reduction neutralization test is the only credible method for measuring actual virus NAbs; however, its feasibility is hampered by drawbacks, such as long turnaround times, low throughput, and the need for laboratory biosafety equipment. To solve these problems, we developed a simple and rapid MV‐NAb detection system using lentivirus‐based virus‐like particles incorporated with the NanoLuc fragment peptide HiBiT comprising the MV fusion protein and hemagglutinin on their exterior surface. Overall, this simple, safe, and rapid method could be used to detect MV NAbs.
Funder
Japan Society for the Promotion of Science
Reference8 articles.
1. Impact of COVID-19 pandemic on routine immunization
2. Seroprevalence to Measles Virus after Vaccination or Natural Infection in an Adult Population, in Italy
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4. Development of a Novel Efficient Fluorescence-Based Plaque Reduction Microneutralization Assay for Measles Virus Immunity
5. Rapid quantitative screening assay for SARS-CoV-2 neutralizing antibodies using HiBiT-tagged virus-like particles