Generation of a photocontrollable recombinant bovine parainfluenza virus type 3

Author:

Okura Takashi1ORCID,Tahara Maino1,Otsuki Noriyuki1,Sato Moritoshi23,Takeuchi Kaoru4,Takeda Makoto1ORCID

Affiliation:

1. Department of Virology 3 National Institute of Infectious Diseases Tokyo Japan

2. Graduate School of Arts and Sciences The University of Tokyo Tokyo Japan

3. Kanagawa Institute of Industrial Science and Technology Kanagawa Japan

4. Laboratory of Environmental Microbiology, Faculty of Medicine University of Tsukuba Tsukuba Ibaraki Japan

Abstract

AbstractBovine parainfluenza virus type 3 (BPIV3) is a promising vaccine vector against various respiratory virus infections, including the human PIV3, respiratory syncytial virus, and severe acute respiratory syndrome‐coronavirus 2 infections. In this study, we combined the Magnet system and reverse genetic approach to generate photocontrollable BPIV3. An optically controllable Magnet gene was inserted into the H2 region of the BPIV3 large protein gene, which encodes an RNA‐dependent RNA polymerase. The generated photocontrollable BPIV3 grew in specific regions of the cell sheet only when illuminated with blue light, suggesting that spatiotemporal control can aid in safe clinical applications of BPIV3.

Funder

Japan Society for the Promotion of Science

Ministry of Health, Labour and Welfare

Research Foundation for Opto-Science and Technology

Naito Foundation

Publisher

Wiley

Subject

Virology,Immunology,Microbiology

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