Reverse transcription polymerase chain reaction-based method for selectively detecting vegetative cells of toxigenicClostridium difficile

Author:

Senoh Mitsutoshi1,Kato Haru1,Murase Tomoko2,Hagiya Hideharu3,Tagashira Yasuaki4,Fukuda Tadashi1,Iwaki Masaaki1,Yamamoto Akihiko1,Shibayama Keigo1

Affiliation:

1. National Institute of Infectious Diseases; Musashimurayama Tokyo Japan

2. Tsuyama Chuo Hospital; Tsuyama Okayama Japan

3. Okayama University Graduate School of Medicine; Dentistry and Pharmaceutical Sciences; Okayama Okayama Japan

4. Tokyo Metropolitan Tama Medical Center; Fuchu Tokyo Japan

Funder

The Ministry of Health, Labor, and Welfare, Japan

Publisher

Wiley

Subject

Virology,Immunology,Microbiology

Reference21 articles.

1. Vital signs: preventing Clostridium difficile infections;Centers for Disease Control and Prevention (CDC);Morb Mortal Wkly Rep,2012

2. The role of toxin A and toxin B in Clostridium difficile infection;Kuehne;Nature,2010

3. Toxin B is essential for virulence of Clostridium difficile;Lyras;Nature,2009

4. Clostridium difficile binary toxin CDT: Mechanism, epidemiology, and potential clinical importance;Gerding;Gut Microbes,2014

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