PbHsfC1a‐coordinates ABA biosynthesis and H2O2 signalling pathways to improve drought tolerance in Pyrus betulaefolia

Author:

Zhang Feng1,Wang Chunmeng1,Yao Jia‐Long2ORCID,Xing Caihua1ORCID,Xu Kang1,Zhang Zan1,Chen Qiming1,Qiao Qinghai1ORCID,Dong Huizhen1,Han Chenyang1,Lin Likun1ORCID,Zhang Shaoling1ORCID,Huang Xiaosan1ORCID

Affiliation:

1. State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing China

2. The New Zealand Institute for Plant and Food Research Limited Auckland New Zealand

Abstract

SummaryAbiotic stresses have had a substantial impact on fruit crop output and quality. Plants have evolved an efficient immune system to combat abiotic stress, which employs reactive oxygen species (ROS) to activate the downstream defence response signals. Although an aquaporin protein encoded by PbPIP1;4 is identified from transcriptome analysis of Pyrus betulaefolia plants under drought treatments, little attention has been paid to the role of PIP and ROS in responding to abiotic stresses in pear plants. In this study, we discovered that overexpression of PbPIP1;4 in pear callus improved tolerance to oxidative and osmotic stresses by reconstructing redox homeostasis and ABA signal pathways. PbPIP1;4 overexpression enhanced the transport of H2O2 into pear and yeast cells. Overexpression of PbPIP1;4 in Arabidopsis plants mitigates the stress effects caused by adding ABA, including stomatal closure and reduction of seed germination and seedling growth. Overexpression of PbPIP1;4 in Arabidopsis plants decreases drought‐induced leaf withering. The PbPIP1;4 promoter could be bound and activated by TF PbHsfC1a. Overexpression of PbHsfC1a in Arabidopsis plants rescued the leaf from wilting under drought stress. PbHsfC1a could bind to and activate AtNCED4 and PbNCED4 promoters, but the activation could be inhibited by adding ABA. Besides, PbNCED expression was up‐regulated under H2O2 treatment but down‐regulated under ABA treatment. In conclusion, this study revealed that PbHsfC1a is a positive regulator of abiotic stress, by targeting PbPIP1;4 and PbNCED4 promoters and activating their expression to mediate redox homeostasis and ABA biosynthesis. It provides valuable information for breeding drought‐resistant pear cultivars through gene modification.

Funder

National Basic Research Program of China

National Natural Science Foundation of China

Publisher

Wiley

Subject

Plant Science,Agronomy and Crop Science,Biotechnology

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