Habitat fragmentation affects climate adaptation in a forest herb

Abstract

Abstract Climate change and the resulting increased drought frequencies pose considerable threats to forest herb populations, particularly where additional environmental challenges jeopardize responses to selection. Specifically, habitat fragmentation may hamper climate adaptation by altering the distribution of adaptive genetic variation and may also induce evolutionary changes in mating systems. To assess how habitat fragmentation disrupts climate adaptation, we conducted a common garden experiment with Primula elatior offspring originating from 24 populations sampled along a latitudinal gradient with varying climate and landscape characteristics. We then quantified a range of vegetative, regulatory and reproductive traits under distinct soil moisture regimes to evaluate imprints of local adaptation and phenotypic plasticity. Additionally, we conducted a more extensive field campaign in 60 populations along the same latitudinal gradient to evaluate the potential evolutionary breakdown of reciprocal herkogamy. For large, connected populations, our results demonstrated an evolutionary shift from a strategy in southern populations that seems aligned with drought avoidance—where plants minimize their exposure to dry conditions and optimize photosynthesis—to a drought tolerance strategy in northern populations, where plants are adapted to function despite water scarcity. However, habitat fragmentation disrupted climate clines and the adaptive responses to drought stress in key traits related to growth, biomass allocation and water regulation. Additionally, our findings indicate the onset of evolutionary breakdown in reciprocal herkogamy and divergence in other key flower traits. The disruption of climate clines, drought responses and adaptations in mating systems contributed to a substantially diminished flowering investment across the distribution range, with the most pronounced effects observed in southern fragmented populations. Synthesis. We present novel empirical evidence of how habitat fragmentation disrupts climate adaptation and drought tolerance in a wide range of traits along the range of the forest herb Primula elatior. These findings emphasize the need to account for habitat fragmentation while designing effective conservation strategies in order to preserve and restore resilient meta‐populations of forest herbs amidst ongoing global changes.