Affiliation:
1. Department of Molecular Neurobiology Institute for Developmental Research, Aichi Developmental Disability Center Kasugai Japan
2. Department of Neurochemistry Nagoya University Graduate School of Medicine Nagoya Japan
Abstract
AbstractThe mediator complex comprises multiple subcellular subunits that collectively function as a molecular interface between RNA polymerase II and gene‐specific transcription factors. Recently, genetic variants to one subunit of the complex, known as MED13L (mediator complex subunit 13 like), have been implicated in syndromic intellectual disability and distinct facial features, frequently accompanied by congenital heart defects. We investigated the impact of five disease‐associated MED13L variants on the subcellular localization and biochemical stability of MED13L protein in vitro and in vivo. In overexpression assays using cortical neurons from embryonic mouse cerebral cortices transduced by in utero electroporation‐mediated gene transfer, we found that mouse orthologues of human MED13L‐p.P866L and ‐p.T2162M missense variants accumulated in the nucleus, while the p.S2163L and p.S2177Y variants were diffusely distributed in the cytoplasm. In contrast, we found that the p.Q1922* truncation variant was barely detectable in transduced cells, a phenotype reminiscent of this variant that results in MED13L haploinsufficiency in humans. Next, we analyzed these variants for their effects on neuronal migration, dendritic growth, spine morphology, and axon elongation of cortical neurons in vivo. There, we found that overexpression of the p.P866L variant resulted in reduced number and length of dendrites of cortical layer II/III pyramidal neurons. Furthermore, we show that mMED13L‐knockdown abrogated dendritic growth in vivo, and this effect was significantly rescued by co‐electroporation of an RNAi‐resistant mMED13L, but weakly by the p.T2162M variant, and not at all by the p.S2163L variant. However, overexpression of the p.S2163L variant inhibited mature dendritic spine formation in vivo. Expression of each of the 5 variants did not affect neuronal cell migration and callosal axon elongation in vivo. Taken together, our results demonstrate that MED13L expression is relevant to corticogenesis and influences the dendritic branching characteristics of cortical excitatory neurons. Our study also suggests that disease‐associated MED13L variants may directly cause morphological and functional defects in cortical neurons in different ways.image
Funder
Japan Agency for Medical Research and Development
Japan Society for the Promotion of Science
Subject
Cellular and Molecular Neuroscience,Biochemistry
Cited by
1 articles.
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