Selection and validation of reference genes for <scp>qPCR</scp> analysis of differentiation and maturation of <scp>THP</scp> ‐1 cells into <scp>M1</scp> macrophage‐like cells-Reference-Cited by-同舟云学术

Selection and validation of reference genes for qPCR analysis of differentiation and maturation of THP ‐1 cells into M1 macrophage‐like cells

Published:2022-10-26 Issue:10 Volume:100 Page:822-829
ISSN:0818-9641
Container-title:Immunology & Cell Biology
language:en
Short-container-title:Immunol Cell Biol

Author:

Ren Guoqiang¹^ORCID,Juhl Morten²,Peng Qiuyue¹,Fink Trine¹,Porsborg Simone Riis¹^ORCID

Affiliation:

1. Regenerative Medicine Group, Department of Health Science and Technology Aalborg University Fredrik Bajers Vej 3B 9220 Aalborg Denmark

2. Cardiology Stem Cell Centre, The Centre for Cardiac, Vascular, Pulmonary and Infectious Diseases Copenhagen University Hospital Rigshospitalet Denmark

Funder

Diabetesforeningen

China Scholarship Council

Publisher

Wiley

Subject

Cell Biology,Immunology,Immunology and Allergy

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1111/imcb.12590

Reference31 articles.

1. The human leukemia cell line, THP-1: A multifacetted model for the study of monocyte-macrophage differentiation

2. Monocyte-derived macrophages and myeloid cell lines as targets of HIV-1 replication and persistence

3. M1 and M2 macrophages derived from THP-1 cells differentially modulate the response of cancer cells to etoposide

4. Standardized protocols for differentiation of THP-1 cells to macrophages with distinct M(IFNγ+LPS), M(IL-4) and M(IL-10) phenotypes

5. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

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