Affiliation:
1. Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche‐Università di Palermo Palermo Italy
Abstract
AbstractThe effects of lipopolysaccharide (LPS) on Mif (macrophage migration inhibitory factor) gene expression in the pharynx (haemapoetic tissue) of Ciona robusta were investigated using quantitative reverse‐transcription PCR (qRT‐PCR) and in situ hybridisation (ISH). To verify the induction of an inflammatory response in the pharynx, a qRT‐PCR analysis was performed to evaluate the change in the expression of proinflammatory marker genes such as Mbl, Ptx‐like, Tnf‐α and Nf‐kb, which were shown to be upregulated 1 h post LPS challenge. The change in the expression of the two Mif paralogs in the pharynx was assessed before and after stimulation, and qRT‐PCR and ISH results showed that, although Mif2 and Mif2 were expressed in clusters of haemocytes in pharynx vessels, only Mif1 expression increased after LPS stimulation. This indicates that the Mif genes are differently regulated and respond to different ambient inputs that need further analysis.
Funder
Università degli Studi di Palermo
Subject
Aquatic Science,Ecology, Evolution, Behavior and Systematics