First report <i>Calonectria hawksworthii</i> as an aetiological agent of fruit rot of cocoa in Brazil-Reference-Cited by-同舟云学术

First report Calonectria hawksworthii as an aetiological agent of fruit rot of cocoa in Brazil

Published:2024-03 Issue:2 Volume:172 Page:
ISSN:0931-1785
Container-title:Journal of Phytopathology
language:en
Short-container-title:Journal of Phytopathology

Author:

de Melo Maruzanete Pereira¹^ORCID,de Sousa Martins Sara¹,de Souza Soares Poliana Prates²,Mesquita Naasoom Luiz Santos²^ORCID,Santos Armínio²,de Novaes Quelmo Silva²,Alves Vitória Cristina Santiago³,Bezerra José Luiz⁴,Bezerra Jadson Diogo Pereira⁵

Affiliation:

1. Centro de Ciências Agrárias Universidade Federal Do Piauí Teresina Piauí Brazil

2. Departamento de Fitotecnia e Zootecnia Universidade Estadual Do Sudoeste da Bahia Vitória da Conquista Bahia Brazil

3. Centro de Biociências Universidade Federal de Pernambuco Recife Pernambuco Brazil

4. Centro de Ciências Agrárias Universidade Federal Do Recôncavo da Bahia Cruz das Almas Bahia Brazil

5. Instituto de Patologia Tropical e Saúde Pública Universidade Federal de Goiás Goiânia Goiás Brazil

Abstract

AbstractCacao (Theobroma cacoa L.) is an important tropical fruit tree used in chocolate production. Among the biotic problems interfering with fruit production, phytopathogenic fungi stand out. During a visit to cocoa production areas in the municipality of Medicilândia, Pará, Brazil (Amazon region), we observed cocoa fruits with dark lesions and greyish‐white cottony mycelia growth. In order to determine the aetiological agent's identity, we extracted DNA and amplified part of the TEF‐1α and RBP2 genes and compared them with sequences in GenBank using the BLASTn tool. Based on molecular results and phylogenetic analysis, the isolates were identified as Calonectria hawksworthii. Inoculations in cacao fruits showed symptoms after 6 days and were similar to those collected in the field, confirming the postulates. This study is the first report of Ca. hawksworthii causing cocoa bean rot worldwide.

Publisher

Wiley

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1111/jph.13305

Reference31 articles.

1. Diversity and potential impact of Calonectria species in Eucalyptus plantations in Brazil

2. Podridão de frutos do cacaueiro causada por Cylindrocladium scoparium Morg;Bastos C. N.;Agrotrópica,2010

3. New endophytic Toxicocladosporium species from cacti in Brazil, and description of Neocladosporium gen. nov.

4. A method for designing primer sets for speciation studies in filamentous ascomycetes

5. Identification of pathogens causing rhizome rot of the east Indian lotus;Chen L. S.;Plant Protection Bulletin,2005