Identification and clinical validation of the role of anoikis‐related genes in diabetic foot

Author:

Su Nan12,Wang Jiwei12,Zhang Hengrui12,Jin Haoyong12,Miao Baojian12,Zhao Jiangli12,Liu Xuchen12,Li Chao12,Wang Xinyu12,Yang Ning12

Affiliation:

1. Department of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain‐Inspired Science Shandong University Jinan China

2. School of Medicine, Cheeloo College of Medicine Shandong University Jinan China

Abstract

AbstractThis study aims to investigate the role of anoikis‐related genes in diabetic foot (DF) by utilizing bioinformatics analysis to identify key genes associated with anoikis in DF. We selected the GEO datasets GSE7014, GSE80178 and GSE68183 for the extraction and analysis of differentially expressed anoikis‐related genes (DE‐ARGs). GO analysis and KEGG analysis indicated that DE‐ARGs in DF were primarily enriched in apoptosis, positive regulation of MAPK cascade, anoikis, focal adhesion and the PI3K‐Akt signalling pathway. Based on the LASSO and SVM‐RFE algorithms, we identified six characteristic genes. ROC curve analysis revealed that these six characteristic genes had an area under the curve (AUC) greater than 0.7, indicating good diagnostic efficacy. Expression analysis in the validation set revealed downregulation of CALR in DF, consistent with the training set results. GSEA results demonstrated that CALR was mainly enriched in blood vessel morphogenesis, endothelial cell migration, ECM‐receptor interaction and focal adhesion. The HPA database revealed that CALR was moderately enriched in endothelial cells, and CALR was found to interact with 63 protein‐coding genes. Functional analysis with DAVID suggested that CALR and associated genes were enriched in the phagosome component. CALR shows promise as a potential marker for the development and treatment of DF.

Funder

Natural Science Foundation of Shandong Province

National Natural Science Foundation of China

Publisher

Wiley

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