Cryopreservation effect on sperm viability, mitochondrial membrane potential, acrosome integrity and sperm capacitation of alpaca spermatozoa detected by imaging flow cytometry

Author:

Santiani Alexei1,Juárez Javier1,Allauca Pablo1,Roman Brian2,Ugarelli Alejandra2ORCID,Evangelista‐Vargas Shirley2

Affiliation:

1. Laboratory of Animal Reproduction, Faculty of Veterinary Medicine Universidad Nacional Mayor de San Marcos (UNMSM) San Borja Peru

2. Laboratory of Reproductive and Cellular Biotechnologies, Faculty of Veterinary and Biological Sciences Universidad Científica del Sur (UCSUR) Lima Peru

Abstract

AbstractEighty‐five sperm samples were cryopreserved and SYBR14/PI, MitoTracker Deep Red FM, FITC‐PSA/PI and chlortetracycline were used for imaging flow cytometry evaluation of sperm viability, mitochondrial membrane potential (MMP), acrosome integrity and sperm capacitation, respectively. Sperm motility was also registered. Sperm motility (46.1 ± 7.7 vs. 24.1% ± 6.5%), sperm viability (49.8 ± 11.5 vs. 32.3% ± 9.6%) and high MMP (49.8% ± 12.4% vs. 34.9% ± 9.9%) decreased significantly (p < .05) during cryopreservation process, in contrast to acrosome‐reacted in viable spermatozoa (1.0% ± 1.6% vs. 1.0% ± 1.0%) and sperm capacitation (10.0 ± 9.8 vs. 8.2% ± 12.4%) that were similar (p > .05) before and after cryopreservation. Positive correlations were found between sperm motility versus high MMP (r = .63), sperm motility versus sperm viability (r = .67) and sperm viability versus high MMP (r = .88). In conclusion, cryopreservation of alpaca spermatozoa is related to a decrease in sperm motility, sperm viability and high MMP, meanwhile acrosome integrity and sperm capacitation are not affected.

Publisher

Wiley

Subject

Endocrinology,Animal Science and Zoology,Biotechnology

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