Road‐traffic‐related air pollution contributes to skin barrier alteration and growth defect of sensory neurons

Author:

Le Gall‐Lanotto Christelle12ORCID,Verdin Anthony3,Cazier Fabrice4,Bataille‐Savattier Adeline1,Guéré Christelle5,Dorr Marie M.5,Fluhr Joachim W.1267,Courcot Dominique3,Vié Katell5,Misery Laurent12

Affiliation:

1. Univ Brest, LIEN Brest France

2. Department of Dermatology University Hospital Brest France

3. EA4492‐Unit of Environmental Chemistry And Interactions With Living Organisms (UCEIV), SFR Condorcet FR CNRS 3417 Université du Littoral Côte d'Opale Dunkerque France

4. Common Center of Measurements (CCM) Université du Littoral Côte d'Opale Dunkerque France

5. Laboratoires Clarins Pontoise France

6. Charité‐Universitätsmedizin Berlin Institute of Allergology Berlin Germany

7. Institute of Allergology and Immunology, Fraunhofer Institute for Translational Medicine and Pharmacology ITMP Berlin Germany

Abstract

AbstractThe effects of air pollution on health are gaining increasing research interest with limited data on skin alterations available. It was suggested that air pollution is a trigger factor for sensitive skin (SS). However, this data was based on surveys with a lack of experimental data. SS is related to altered skin nerve endings and cutaneous neurogenic inflammation. TTe present study was to assess the in vitro effect of particulate matter (PM) on epidermis and nerve ending homeostasis. PM samples were collected according to a validated protocol. Reconstructed human epidermis (RHE, Episkin®) was exposed to PM and subsequently the supernatants were transferred to a culture of PC12 cells differentiated into sensory neurons (SN). Cell viability, axonal growth and neuropeptide‐release were measured. The modulation of the expression of different inflammatory, keratinocytes differentiation and neurites growth markers was assessed. PM samples contained a high proportion of particles with a size below 1 μm and a complex chemical composition. Transcriptomic and immunohistochemical analyses revealed that PM altered keratinocytes terminal differentiation and induced an inflammatory response. While viability and functionality of the SN were not modified, their outgrowth was significantly decreased after incubation with PM‐exposed Episkin® supernatants. This was closely related to the modification of nerve growth factor/semaphorin 3A balance. This study showed that air pollutants have negative effects on keratinocytes and sensory nerve endings including inflammatory responses. These effects are probably involved in the SS pathophysiology and might be involved in inflammatory skin disorders.

Publisher

Wiley

Subject

Dermatology,Molecular Biology,Biochemistry

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3