Affiliation:
1. School of Medicine Yunnan University Kunming China
2. Department of Prosthodontics of Kunming Medical University Stomatology Hospital of Kunming Medical University Kunming China
3. Department of Oral and Maxillofacial Surgery of Kunming Medical University Stomatology Hospital of Kunming Medical University Kunming China
4. Department of General Dentistry of Kunming Medical University Stomatology Hospital of Kunming Medical University Kunming China
Abstract
AbstractObjectiveThe aim of this study was to investigate the molecular mechanism by which the transcription factor ETS1 regulates N‐myc downstream regulatory gene 1 (NDRG1) to provide a new theoretical basis for the study of oral squamous cell carcinoma (OSCC).MethodsIn this study, eight human OSCC and paraneoplastic samples were collected. The expressions of NDRG1, ETS1, and Ki67 were detected by immunohistochemistry; apoptosis was detected by tdt‐mediated dUTP notched end labeling; cell migration and invasion were detected by Transwell; quantitative real‐time PCR was performed to detect the expression of NDRG1; RNA‐binding protein immunoprecipitation (RIP) assays detected NDRG1 expression; immunofluorescence assays detected ETS1 expression.ResultsNDRG1 and ETS1 expression was significantly upregulated in cancer tissues and CAL‐27 and SCC‐6 cells. Knockdown of NDRG1 and ETS1 inhibited cell proliferation, migration, invasion, cloning, and EMT while promoting apoptosis and inhibited tumor development; ETS1 positively regulated NDRG1 expression; Finally, overexpression of NDRG1 in vivo and in vitro reversed the effect of ETS1 knockdown on CAL‐27 and SCC‐6 cells.ConclusionsETS1 positively regulates the expression of NDRG1 and promotes OSCC. Therefore, ETS1 may serve as a new target for the clinical diagnosis and treatment of OSCC.
Subject
General Dentistry,Otorhinolaryngology
Cited by
1 articles.
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