Neuronal plasticity in hippocampal neurons due to chronic mild stress and after stress removal in postnatal chicks

Abstract

AbstractThe avian dorsomedial surface of the cerebral hemisphere is occupied by the hippocampal complex (HCC), which plays an important role in learning, memory, cognitive functions, and regulating instinctive behavior patterns. The objective of the study was to evaluate the effect of chronic mild stress (CMS) in 4, 6, and 8 weeks and after chronic stress removal (CSR) in 6 and 8 weeks, on neuronal plasticity in HCC neurons of chicks through the Golgi–Cox technique. Further, behavioral study and open field test were conducted to test of exploration or of anxiety. The study revealed that the length of CMS and CSR groups shows a similar pattern as in nonstressed (NS) chicks, while weight shows nonsignificant decrease due to CMS as compared to NS and after CSR. The behavioral test depicts that the CMS group took more time to reach the food as compared to the NS and CSR groups. Due to CMS, the dendritic field of multipolar neurons shows significant decrease in 4 weeks, but in 6‐ and 8‐week‐old chicks, the multipolar, pyramidal, and stellate neurons depict significant decrease, whereas after CSR all neurons show significant increase in 8‐week‐old chicks. In 4‐ and 8‐week‐old chicks, all neurons depict significant decrease in their spine number, whereas in 6 weeks only multipolar neurons show significant decrease, but after CSR significant increase in 8‐week‐old chicks was observed. The study revealed that HCC shows continuous neuronal plasticity, which plays a significant role in normalizing and re‐establishing the homeostasis in animals to survive.