Expression and purification of antimicrobial peptide AP2 using SUMO fusion partner technology inEscherichia coli

Author:

Mo Q.12,Fu A.13,Lin Z.1,Wang W.4,Gong L.1,Li W.1

Affiliation:

1. Key Laboratory of Animal Molecular Nutrition of Education of Ministry; College of Animal Sciences, Zhejiang University; Hangzhou China

2. College of Biosystems Engineering and Food Science, Zhejiang University; Hangzhou China

3. Institute of Biology, Westlake Institute for Advanced Study; Westlake University; Hangzhou Zhejiang Province China

4. Linyi Institute for Food and Drug Control; Linyi Shandong China

Funder

National Support Program for Science and Technology for rural development in China

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology

Reference34 articles.

1. Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?;Brogden;Nat Rev Microbiol,2005

2. SUMO fusion technology for difficult-to-express proteins;Butt;Protein Expres Purif,2005

3. Apidaecins: antibacterial peptides from honeybees;Casteels;EMBO J,1989

4. Biodiversity of apidaecin-type peptide antibiotics - prospects of manipulating the antibacterial spectrum and combating acquired-resistance;Casteels;J Biol Chem,1994

5. Recombinant Nogo-66 via soluble expression with SUMO fusion in Escherichia coli inhibits neurite outgrowth in vitro;Dai;Appl Microbiol Biotechnol,2015

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