Affiliation:
1. National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai China
2. University of Chinese Academy of Sciences Beijing China
3. Chinese Academy of Sciences (CAS) and CAS John Innes Centre of Excellence for Plant and Microbial Sciences Shanghai China
Abstract
SUMMARYAir humidity significantly impacts plant physiology. However, the upstream elements that mediate humidity sensing and adaptive responses in plants remain largely unexplored. In this study, we define high humidity‐induced cellular features of Arabidopsis plants and take a quantitative phosphoproteomics approach to obtain a high humidity‐responsive landscape of membrane proteins, which we reason are likely the early checkpoints of humidity signaling. We found that a brief high humidity exposure (i.e., 0.5 h) is sufficient to trigger extensive changes in membrane protein abundance and phosphorylation. Enrichment analysis of differentially regulated proteins reveals high humidity‐sensitive processes such as ‘transmembrane transport’, ‘response to abscisic acid’, and ‘stomatal movement’. We further performed a targeted screen of mutants, in which high humidity‐responsive pathways/proteins are disabled, to uncover genes mediating high humidity sensitivity. Interestingly, ethylene pathway mutants (i.e., ein2 and ein3eil1) display a range of altered responses, including hyponasty, reactive oxygen species level, and responsive gene expression, to high humidity. Furthermore, we observed a rapid induction of ethylene biosynthesis genes and ethylene evolution after high humidity treatment. Our study sheds light on the potential early signaling events in humidity perception, a fundamental but understudied question in plant biology, and reveals ethylene as a key modulator of high humidity responses in plants.
Subject
Cell Biology,Plant Science,Genetics
Cited by
1 articles.
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