T cells from chronic bone infection show reduced proliferation and a high proportion of CD28− CD4 T cells

Author:

Kumar G1,Roger P-M12,Ticchioni M3,Trojani C4,Bernard de Dompsur R4,Bronsard N4,Carles M5,Bernard E2

Affiliation:

1. Unité 576, Institut National de la Santé et de la Recherche Médicale, Nice, France

2. Service d'Infectiologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia-Antipolis, Hopital L'Archet 1, Nice, France

3. Laboratoire d'Immunologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia-Antipolis, Hopital L'Archet 1, Nice, France

4. Orthopédie-Traumatologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia-Antipolis, Hopital L'Archet 1, Nice, France

5. Anesthésie-Réanimation, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia-Antipolis, Hopital L'Archet 1, Nice, France

Abstract

Summary Chronic bone infection is associated with bone resorption. From animal studies, CD3/CD28-activated T cells are known to enhance osteoclastogenesis and bone resorption. Because CD28 is expressed constitutively on T cells and its expression is down-regulated by chronic exposure to the inflammatory environment, we characterized co-stimulatory molecule expression on T cells from chronically infected patients. We used cytofluorometric techniques to phenotypically characterize T cells, its co-stimulatory molecules and perforin secretion from infected and non-infected human bones. Chronic bone infection was defined as infection lasting for more than a month. We show a higher T cell activation [human leucocyte antigen D-related (HLA-DR+)] in infected compared to non-infected bones: median being 16 versus 7%, P = 0·009 for CD4 T cells, and 33 versus 15%, P = 0·038 for CD8 T cells, respectively. However, T cell proliferation (Ki67+) was lower for CD8 T cells in infected bones: 26 versus 34%, P = 0·045. In contrast, we detected no difference in apoptosis and regulatory T cells. In infected bone, we found higher CD28-negative CD4+ T cells compared to non-infected bone: 20 versus 8%, respectively (P = 0·005); this T cell subset had higher CD11b expression and perforin secretion. Chronically infected human bones are characterized by an increase of CD28-negative CD4+ T cells, indicating long-term activated cells with cytotoxic ability. Therefore, this alteration of co-stimulatory molecules may modify interactions with osteoclasts and impact bone resorption.

Funder

Infectiopole Sud and Recherche Et Developpement en Pathologie Infectieuse et Tropicale

Publisher

Oxford University Press (OUP)

Subject

Immunology,Immunology and Allergy

Reference38 articles.

1. The incidence of sepsis after total hip replacement arthroplasty;Nasser;Semin Arthroplasty,1994

2. Osteomyelitis;Lew;N Engl J Med,1997

3. Osteomyelitis;Lew;Lancet,2004

4. Role of bacterial proteinases in matrix destruction and modulation of host responses;Potempa;Periodontology,2000

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