The kiwifruit amyloplast proteome (kfALP): a resource to better understand the mechanisms underlying amyloplast biogenesis and differentiation

Author:

Li Ang1ORCID,Lin Jiajia1ORCID,Zeng Zhebin1ORCID,Deng Zhiping2ORCID,Tan Jinjuan2ORCID,Chen Xiaoya1ORCID,Ding Gang1ORCID,Zhu Man1ORCID,Xu Bin1ORCID,Atkinson Ross G.3ORCID,Nieuwenhuizen Niels J.3ORCID,Ampomah‐Dwamena Charles3ORCID,Cheng Yunjiang1ORCID,Deng Xiuxin1ORCID,Zeng Yunliu1ORCID

Affiliation:

1. National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Centre for Citrus Preservation, College of Horticulture and Forestry Science Huazhong Agricultural University Wuhan 430070 P.R. China

2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro‐Products, Institute of Virology and Biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China

3. The New Zealand Institute for Plant and Food Research Ltd (PFR) Private Bag Auckland 92169 New Zealand

Abstract

SUMMARYThe biogenesis and differentiation (B&D) of amyloplasts contributes to fruit flavor and color. Here, remodeling of starch granules, thylakoids and plastoglobules was observed during development and ripening in two kiwifruit (Actinidia spp.) cultivars – yellow‐fleshed ‘Hort16A’ and green‐fleshed ‘Hayward’. A protocol was developed to purify starch‐containing plastids with a high degree of intactness, and amyloplast B&D was studied using label‐free‐based quantitative proteomic analyses in both cultivars. Over 3000 amyloplast‐localized proteins were identified, of which >98% were quantified and defined as the kfALP (kiwifruit amyloplast proteome). The kfALP data were validated by Tandem‐Mass‐Tag (TMT) labeled proteomics in ‘Hort16A’. Analysis of the proteomic data across development and ripening revealed: 1) a conserved increase in the abundance of proteins participating in starch synthesis/degradation during both amyloplast B&D; 2) up‐regulation of proteins for chlorophyll degradation and of plastoglobule‐localized proteins associated with chloroplast breakdown and plastoglobule formation during amyloplast differentiation; 3) constitutive expression of proteins involved in ATP supply and protein import during amyloplast B&D. Interestingly, two different pathways of amyloplast B&D were observed in the two cultivars. In ‘Hayward’, significant increases in abundance of photosynthetic‐ and tetrapyrrole metabolism‐related proteins were observed, but the opposite trend was observed in ‘Hort16A’. In conclusion, analysis of the kfALP provides new insights into the potential mechanisms underlying amyloplast B&D with relevance to key fruit quality traits in contrasting kiwifruit cultivars.

Funder

National Natural Science Foundation of China

Earmarked Fund for China Agriculture Research System

Publisher

Wiley

Subject

Cell Biology,Plant Science,Genetics

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3