Next-generation sequencing can revealin vitro-generated PCR crossover products: some artifactual sequences correspond to HLA alleles in the IMGT/HLA database-Reference-Cited by-同舟云学术

Next-generation sequencing can revealin vitro-generated PCR crossover products: some artifactual sequences correspond to HLA alleles in the IMGT/HLA database

Published:2013-12-19 Issue:1 Volume:83 Page:32-40
ISSN:0001-2815
Container-title:Tissue Antigens
language:en
Short-container-title:Tissue Antigens

Author:

Holcomb C. L.¹,Rastrou M.¹,Williams T. C.²,Goodridge D.³,Lazaro A. M.⁴,Tilanus M.⁵,Erlich H. A.¹²

Affiliation:

1. Department of Human Genetics; Roche Molecular Systems, Inc.; Pleasanton CA USA

2. Children's Hospital Oakland Research Institute; Oakland CA USA

3. Conexio Genomics; Perth Australia

4. Department of Oncology; Georgetown University Medical Center; Washington DC USA

5. Department of Transplantation Immunology, Tissue Typing Laboratory; Maastricht University Medical Centre; Maastricht the Netherlands

Publisher

Wiley

Subject

Genetics,Biochemistry,Immunology,General Medicine,Immunology and Allergy

Link

http://onlinelibrary.wiley.com/wol1/doi/10.1111/tan.12269/fullpdf

Reference19 articles.

1. High-resolution, high-throughput HLA genotyping by next-generation sequencing;Bentley;Tissue Antigens,2009

2. A multi-site study using high-resolution HLA genotyping by next generation sequencing;Holcomb;Tissue Antigens,2011

3. High throughput HLA genotyping using 454 sequencing and the Fluidigm Access Array™ system for simplified amplicon library preparation;Moonsamy;Tissue Antigens,2013

4. The IMGT/HLA database;Robinson;Nucleic Acids Res,2013

5. Primer-directed enzymatic amplification of DNA with a thermal stable DNA polymerase;Saiki;Science,1988

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