Enzymatic Resolution of Racemic Amines.

Author:

KITAGUCHI HIROSHI,FITZPATRICK PAUL A.,HUBER JOEL E.,KLIBANOV ALEXANDER M.

Publisher

Wiley

Subject

History and Philosophy of Science,General Biochemistry, Genetics and Molecular Biology,General Neuroscience

Reference10 articles.

1. Asymmetric transformations catalyzed by enzymes in organic solvents

2. Enzyme-catalyzed processes in organic solvents.

3. Enzymic resolution of racemic amines: crucial role of the solvent

4. 4. In addition to those mentioned in the text, lipases from the following cultures were tested:Aspergillus niger, Humiloca lanuginosa, Geotrichum candidum, Rhizopus japonicus, Rhizopus delemar, Penicillium cyclopium, Penicillium roqueforti, Rhizopus arrhizus, Candida lipolitica, andRhizopus iavanicus.

5. 5. Here, 100 mg/mL enzyme suspensions in anhydrous octane containing 200 mM trifluoroethyl butyrate and 100 mM amine were shaken at 45oC and 250 rpm; the reactions were followed by capillary gas chromatography on the basis of the formation of the amide product. No appreciable amidation reaction was detected without enzymes.

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