Is it safe to use ovarian follicular fluid from cows seropositive for brucellosis in the production of in vitro embryos?

Author:

Andrade Rafaella Silva1ORCID,Custódio Dircéia Aparecida da Costa1ORCID,Silva Fernandes do Vale Leonardo1ORCID,Oliveira Érika Aparecida1ORCID,Lage Andrey Pereira2ORCID,Dorneles Elaine Maria Seles1ORCID

Affiliation:

1. Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária Universidade Federal de Lavras Lavras Minas Gerais Brazil

2. Departamento de Medicina Veterinária Preventiva, Escola de Veterinária Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil

Abstract

AbstractAnimal reproduction biotechniques are important tools for the technological advancement of livestock, as they allow the selection of the reproductive potential of superior quality females and males; however, infectious diseases that have a predilection for the reproductive system can be a hindrance for the use of these technologies. Therefore, the present study aimed to detect Brucella spp. in the ovarian follicular fluid of brucellosis‐positive bovine cows. A total of 47 bovine ovarian follicular fluid aspirates from cows, positive in tests for brucellosis and from Brucella‐positive herd, were submitted to PCR. The primers used in the PCR were specific to the genus Brucella (bcsp31 gene). All 47 bovine aspirates were negative for Brucella spp. 0.00% (95% CI: 0.00–4.00%). Our results demonstrated that Brucella spp. was absent in the ovarian follicular fluid from seropositive cows, which indicates that Brucella spp.‐infected cows could be used for reproductive biotechnologies carried out with follicular aspirates. Future studies are needed to more precisely evaluate the feasibility and safety of using these oocytes from brucellosis‐seropositive cows to transfer embryos to heifers/cows not infected by Brucella, aiming to produce calves free of the infection.

Publisher

Wiley

Reference31 articles.

1. Detection of Brucella abortus during in vitro bovine embryo production;Akter A.;Bangladesh Journal of Livestock Research,2020

2. Detection of Brucella melitensis and Brucella abortus by DNA amplification;Baily G. G.;The American Journal of Tropical Medicine and Hygiene,1992

3. Failure to isolate Brucella abortus from embryos or ova from culture-positive superovulated cows

4. Confidence Intervals for a binomial proportion and asymptotic expansions

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