Efficient assembly of long <scp>DNA</scp> fragments and multiple genes with improved nickase‐based cloning and Cre/ <scp> <i>loxP</i> </scp> recombination-Reference-Cited by-同舟云学术

Efficient assembly of long DNA fragments and multiple genes with improved nickase‐based cloning and Cre/ loxP recombination

Published:2022-07-16 Issue:10 Volume:20 Page:1983-1995
ISSN:1467-7644
Container-title:Plant Biotechnology Journal
language:en
Short-container-title:Plant Biotechnology Journal

Author:

Zhao Yanchang¹²³,Han Jingluan¹²³^ORCID,Tan Jiantao¹²³,Yang Yaqian¹³,Li Shuangchun¹³,Gou Yajun¹³,Luo Yuyu¹³,Li Tie¹³,Xiao Wenyu³,Xue Yang¹³,Hao Yu¹³,Xie Xianrong¹²³,Liu Yao‐Guang¹²³^ORCID,Zhu Qinlong¹²³^ORCID

Affiliation:

1. State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources South China Agricultural University Guangzhou China

2. Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou China

3. College of Life Sciences South China Agricultural University Guangzhou China

Funder

National Natural Science Foundation of China

Publisher

Wiley

Subject

Plant Science,Agronomy and Crop Science,Biotechnology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1111/pbi.13882

Reference51 articles.

1. Nicking Endonucleases as Unique Tools for Biotechnology and Gene Engineering

2. BglBricks: A flexible standard for biological part assembly

3. An improved method for fast, robust, and seamless integration of DNA fragments into multiple plasmids

4. USERTM friendly DNA engineering and cloning method by uracil excision

5. Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids

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