A complex tissue‐specific interplay between the Arabidopsis transcription factors AtMYB68, AtHB23, and AtPHL1 modulates primary and lateral root development and adaptation to salinity

Author:

Spies Fiorella Paola1ORCID,Perotti María Florencia1ORCID,Cho Yuhan2ORCID,Jo Chang Ig2ORCID,Hong Jong Chan23ORCID,Chan Raquel Lía1ORCID

Affiliation:

1. Instituto de Agrobiotecnología del Litoral, CONICET Universidad Nacional del Litoral, FBCB Colectora Ruta Nacional 168 km 0 3000 Santa Fe Argentina

2. Division of Life Science, Applied Life Science (BK21 Four), Plant Molecular Biology and Biotechnology Research Center Gyeongsang National University Jinju Gyeongnam 52828 South Korea

3. Division of Plant Sciences University of Missouri Columbia South Carolina MO 65211‐7310 USA

Abstract

SUMMARYAdaptation to different soil conditions is a well‐regulated process vital for plant life. AtHB23 is a homeodomain‐leucine zipper I transcription factor (TF) that was previously revealed as crucial for plant survival under salinity conditions. We wondered whether this TF has partners to perform this essential function. Therefore, TF cDNA library screening, yeast two‐hybrid, bimolecular fluorescence complementation, and coimmunoprecipitation assays were complemented with expression analyses and phenotypic characterization of silenced, mutant, overexpression, and crossed plants in normal and salinity conditions. We revealed that AtHB23, AtPHL1, and AtMYB68 interact with each other, modulating root development and the salinity response. The encoding genes are coexpressed in specific root tissues and at specific developmental stages. In normal conditions, amiR68 silenced plants have fewer initiated roots, the opposite phenotype to that shown by amiR23 plants. AtMYB68 and AtPHL1 play opposite roles in lateral root elongation. Under salinity conditions, AtHB23 plays a crucial positive role in cooperating with AtMYB68, whereas AtPHL1 acts oppositely by obstructing the function of the former, impacting the plant's survival ability. Such interplay supports the complex interaction between these TF in primary and lateral roots. The root adaptation capability is associated with the amyloplast state. We identified new molecular players that through a complex relationship determine Arabidopsis root architecture and survival in salinity conditions.

Funder

Fondo para la Investigación Científica y Tecnológica

Publisher

Wiley

Subject

Cell Biology,Plant Science,Genetics

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