Affiliation:
1. State Key Laboratory of Biocatalysis and Enzyme Engineering Hubei University Wuhan 430062 P.R. China
2. Hubei Key Laboratory of Industrial Biotechnology, School of Life Science Hubei University Wuhan 430062 P.R. China
3. College of Life Science and Technology Hubei Engineering University Xiaogan 432000 P.R. China
4. Guilin Jingcheng Biotechnology Co., Ltd. Guilin 541001 P.R. China
Abstract
AbstractIn the process of bioethanol production, more stable and active cellulase in high temperature condition is required. In this study, syringic acid was applied in cellulase hydrolysis system. At 70°C, TvEG3 activity increased 201.36%, CtBglA activity decreased 72.79% by syringic acid. With syringic acid assisting, TvEG3 thermostability was improved, CtBglA thermostability was reduced. Syringic acid scarcely affected CtCBH. In hydrolysis system with the cellulases containing TvEG3, CtCBH, and CtBglA, the reducing sugar yield improved by 28.37% with syringic acid assisting. With the molecular dynamic simulation in syringic acid system, the backbone root‐mean‐square deviation (RMSD) and the residue root‐mean‐square fluctuation (RMSF) of TvEG3, CtCBH reduced, while the RMSD and RMSF of CtBglA increased. The reduction in the number of secondary structures, especially α‐helix, caused the structure of CtBglA in the presence of syringic acid to collapse at high temperature. More secondary structures in TvEG3 and more α‐helix in CtCBH in the presence of syringic acid make them more stable at high temperatures. These means syringic acid can stabilize TvEG3 and CtCBH structure, destabilize CtBglA structure at high temperature. In summary, this study not only provides insight into cellulase hydrolysis at high temperature with syringic acid assisting but also demonstrates the promoting mechanism of syringic acid.
Subject
Waste Management and Disposal,Agronomy and Crop Science,Renewable Energy, Sustainability and the Environment,Forestry
Cited by
1 articles.
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