Affiliation:
1. Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection Southwest University Chongqing China
2. Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education) Southwest University Chongqing China
3. Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department Guiyang University Guiyang China
Abstract
AbstractZeugodacus cucurbitae Coquillett (Diptera: Tephritidae) is an agriculturally and economically important pest worldwide that has developed resistance to β‐cypermethrin. Glutathione S‐transferases (GSTs) have been reported to be involved in the detoxification of insecticides in insects. We have found that both ZcGSTd6 and ZcGSTd10 were up‐regulated by β‐cypermethrin induction in our previous study, so we aimed to explore their potential relationship with β‐cypermethrin tolerance in this study. The heterologous expression of ZcGSTd6 and ZcGSTd10 in Escherichia coli showed significantly high activities against 1‐chloro‐2,4‐dinitrobenzene (CDNB). The kinetic parameters of ZcGSTd6 and ZcGSTd10 were determined by Lineweaver–Burk. The Vmax and Km of ZcGSTd6 were 0.50 μmol/min·mg and 0.3 mM, respectively. The Vmax and Km of ZcGSTd10 were 1.82 μmol/min·mg and 0.53 mM. The 3D modelling and molecular docking results revealed that β‐cypermethrin exhibited a stronger bounding to the active site SER‐9 of ZcGSTd10. The sensitivity to β‐cypermethrin was significantly increased by 18.73% and 27.21%, respectively, after the knockdown of ZcGSTd6 and ZcGSTd10 by using RNA interference. In addition, the inhibition of CDNB at 50% (IC50) and the inhibition constants (Ki) of β‐cypermethrin against ZcGSTd10 were determined as 0.41 and 0.33 mM, respectively. The Ki and IC50 of β‐cypermethrin against ZcSGTd6 were not analysed. These results suggested that ZcGSTd10 could be an essential regulator involved in the tolerance of Z. cucurbitae to β‐cypermethrin.
Funder
National Key Research and Development Program of China
Southwest University
Cited by
2 articles.
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