Affiliation:
1. Department of Dermatology, China‐Japan Friendship Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing China
2. Department of Dermatology China‐Japan Friendship Hospital Beijing China
Abstract
AbstractNon‐invasive optical examination plays a crucial role in various aspects of dermatology, such as diagnosis, management and research. Multiphoton microscopy uses a unique submicron technology to stimulate autofluorescence (AF), allowing for the observation of cellular structure, assessment of redox status and quantification of collagen fibres. This advanced imaging technique offers dermatologists novel insights into the skin's structure, positioning it as a promising ‘stethoscope’ for future development in the field. This review provides an overview of multiphoton microscopy's principles, technology and application in studying normal skin, tumour and inflammatory diseases, as well as collagen‐related and pigmentary diseases.
Subject
Dermatology,Molecular Biology,Biochemistry