AtVQ25 promotes salicylic acid‐related leaf senescence by fine‐tuning the self‐repression of AtWRKY53

Author:

Tan Qi1ORCID,Zhao Mingming12ORCID,Gao Jingwei1ORCID,Li Ke1ORCID,Zhang Mengwei1ORCID,Li Yunjia1ORCID,Liu Zeting1ORCID,Song Yujia1ORCID,Lu Xiaoyue1ORCID,Zhu Zhengge1ORCID,Lin Rongcheng3ORCID,Yin Pengcheng1ORCID,Zhou Chunjiang1ORCID,Wang Geng1ORCID

Affiliation:

1. Ministry of Education Key Laboratory of Molecular and Cellular Biology, Hebei Research Center of the Basic Discipline of Cell Biology, Hebei Collaboration Innovation Center for Cell Signaling and Environmental Adaptation, Hebei Key Laboratory of Molecular and Cellular Biology, College of Life Sciences Hebei Normal University Shijiazhuang 050024 China

2. College of Chemical Engineering Shijiazhuang University Shijiazhuang 050035 China

3. Institute of Botany Chinese Academy of Sciences Beijing 100093 China

Abstract

ABSTRACTMost mechanistic details of chronologically ordered regulation of leaf senescence are unknown. Regulatory networks centered on AtWRKY53 are crucial for orchestrating and integrating various senescence‐related signals. Notably, AtWRKY53 binds to its own promoter and represses transcription of AtWRKY53, but the biological significance and mechanism underlying this self‐repression remain unclear. In this study, we identified the VQ motif‐containing protein AtVQ25 as a cooperator of AtWRKY53. The expression level of AtVQ25 peaked at mature stage and was specifically repressed after the onset of leaf senescence. AtVQ25‐overexpressing plants and atvq25 mutants displayed precocious and delayed leaf senescence, respectively. Importantly, we identified AtWRKY53 as an interacting partner of AtVQ25. We determined that interaction between AtVQ25 and AtWRKY53 prevented AtWRKY53 from binding to W‐box elements on the AtWRKY53 promoter and thus counteracted the self‐repression of AtWRKY53. In addition, our RNA‐sequencing data revealed that the AtVQ25‐AtWRKY53 module is related to the salicylic acid (SA) pathway. Precocious leaf senescence and SA‐induced leaf senescence in AtVQ25‐overexpressing lines were inhibited by an SA pathway mutant, atsid2, and NahG transgenic plants; AtVQ25‐overexpressing/atwrky53 plants were also insensitive to SA‐induced leaf senescence. Collectively, we demonstrated that AtVQ25 directly attenuates the self‐repression of AtWRKY53 during the onset of leaf senescence, which is substantially helpful for understanding the timing of leaf senescence onset modulated by AtWRKY53.

Funder

Natural Science Foundation of Hebei Province

National Natural Science Foundation of China

Publisher

Wiley

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