The mutual influence of microtubules and the cortical ER on their coordinated organisation

Author:

Pal Lalita1,Belausov Eduard1,Dwivedi Vikas1,Yechezkel Sela1,Sadot Einat1ORCID

Affiliation:

1. The Institute of Plant Sciences Department of Ornamental Plants and Agricultural Biotechnology. The Volcani Institute, ARO Rishon LeZion Israel

Abstract

AbstractThe endoplasmic reticulum (ER) is the largest organelle in terms of membrane content, occupying the entire cytoplasmic volume. It is tethered to the cell cortex through ER‐plasma membrane contact sites (EPCS). Previous studies have shown that EPCSs labelled by VAP27 align with cortical microtubules, and that ER tubules elongate along microtubules. Here, we addressed the question whether this relationship is bidirectional, with EPCSs influencing microtubule organisation. Using TIRF microscopy to track EPCSs and microtubule dynamics simultaneously, we demonstrate that while EPCSs remain stable, microtubules are highly dynamic and can adjust their positioning based on nearby EPCS in Arabidopsis cotyledon epidermis. In lobes of epidermal cells enclosed by two indentations, where microtubules bundle together, EPCSs flank the bundles and exhibit a distinctive arrangement, forming symmetric arcs in relation to the lobe axis. In guard cells, transversely oriented ER tubules co‐align with microtubules. Disrupting microtubules with the drug oryzalin leads to transient guard cells‐ER remodelling, followed by its reorganisation into transverse tubules before microtubule recovery. Taken together our observations suggest, that the positioning of EPCSs and cortical microtubules, can affect each other and the organisation of cortical ER.

Funder

Israel Science Foundation

Publisher

Wiley

Reference42 articles.

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