Affiliation:
1. Department of Dentistry Universidade Federal de Santa Catarina – UFSC Florianópolis SC Brazil
2. Anatomic Pathology Department Universidade de Campinas – UNICAMP Campinas SP Brazil
3. Department of Dentistry Universidad Autónoma de Baja California – UABC Mexicali BC Mexico
4. Department of Pathology Universidade Federal de Santa Catarina – UFSC Florianópolis SC Brazil
Abstract
AbstractObjectiveCarcinoma ex‐pleomorphic adenoma (CEXPA) represents a malignant transformation from a recurrent or primary pleomorphic adenoma (PA), and the immune response may be essential in this process. Therefore, in this study, we aimed to identify and quantify subpopulations of dendritic cells (DCs) in CEXPA, residual PA in CEXPA (rPA), and PA.Materials and MethodsA multicenter study was performed collecting salivary gland tumor (SGT) samples from three Oral and Maxillofacial Pathology Centers. A tissue microarray containing 41 samples of CEXPA and 22 samples of PA was included in this study and submitted to immunohistochemical reactions against CD1a, CD83, CD207, and Ki67 antibodies.ResultsBoth PA and rPA showed a higher quantification of CD207+ and CD83+ cells when compared to CEXPA (p < 0.001 and p < 0.01, respectively). There was also a difference when comparing the cell proliferation index between PA/rPA and CEXPA using the Ki‐67 marker (p = 0.043). However, there was no difference in the DC population regarding clinical parameters such as sex, anatomical location, size, and metastases (p > 0.06).ConclusionsImmunohistochemical profile of DC subpopulations and cell proliferation biomarkers in SGTs can contribute as an important tool in the differentiation of benign and malignant tumors or detection of initial areas with malignant transformation.