Untargeted metabolomic in basil cell cultures – a case study of Ocimum basilicum L. var. minimum Alef.

Author:

Jakovljević Dragana1ORCID,Kruszka Dariusz2,Waligórski Piotr3,Warchoł Marzena3,Skrzypek Edyta3

Affiliation:

1. Faculty of Science, Department of Biology and Ecology University of Kragujevac Kragujevac Serbia

2. Institute of Plant Genetics of the Polish Academy of Sciences Poznań Poland

3. Polish Academy of Sciences The Franciszek Górski Institute of Plant Physiology Kraków Poland

Abstract

AbstractAimsDue to the lack of experimental databases, together with the chemical complexity and the dynamic nature of plants' metabolome, most of the metabolites in complex biological materials (like plant in vitro tissue cultures) are not‐annotated, unidentified metabolites. In this study, a method for further metabolite characterization and classification based on the UPLC‐HESI‐HRMS/MS approach for small‐leaved basil (Ocimum basilicum L. var. minimum Alef.) callus and cell suspension culture is presented.Key resultsA total of 2168 metabolic features were detected, out of which the database for exact mass metabolic profiling for 1949 metabolites is presented here since there is no available database dedicated to O. basilicum. We further focused on secondary metabolites (particularly phenolic compounds). The presence of 60 different phenolic compounds belonging mainly to the groups of flavonoids, glycosides, terpenoids, and phenolic acids is confirmed. By comparing relative abundances of phenolic compounds from callus culture and cell suspension culture, both grown on two types of media, via svd‐PCА, univariate analysis, post‐hoc tests, and heatmapping of metabolites, we provided a practical example of how resources presented here can be further applied in tissue culture‐based basil metabolomics studies.UtilityThis study represents the first approach toward routine targeted investigation of secondary metabolites in basil in vitro cultures and provides various opportunities for new‐generation analyses.

Publisher

Wiley

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