Affiliation:
1. Small Animal Department Ghent University Merelbeke Belgium
2. Department of Internal Medicine, Reproduction and Population Medicine Ghent University Merelbeke Belgium
3. Department of Morphology, Imaging, Orthopedics, Rehabilitation and Nutrition Ghent University Merelbeke Belgium
4. Department of Veterinary and Biosciences Ghent University Merelbeke Belgium
Abstract
AbstractBackgroundCell cycle arrest biomarkers (tissue inhibitor of metalloproteinase‐2 [uTIMP‐2] and insulin‐like growth factor binding protein 7 [uIGFBP7]), and neutrophil gelatinase‐associated lipocalin (NGAL) variables are valuable biomarkers for early diagnosis of acute kidney injury (AKI) in people.ObjectivesTo evaluate uTIMP‐2, uIGFBP7, fractional excretion of NGAL (FeNGAL), and urinary to serum NGAL ratio (u/sNGAL) in healthy dogs, dogs with AKI, dogs with chronic kidney disease (CKD), and critically ill (CI) dogs.AnimalsForty‐two client‐owned dogs (healthy, n = 10; AKI, n = 11; CKD, n = 11; CI, n = 10).MethodsProspective, observational study. After assessment of routine renal biomarkers, stress (uTIMP‐2, uIGFBP7) and damage (NGAL) biomarkers were measured, using ELISA kits, and normalized to urinary creatinine (uCr).ResultsNormalized uTIMP‐2 and [uTIMP‐2] × [uIGFBP7]/uCr were significantly higher in the AKI group (median 151.9 [range, 2.2‐534.2] and 62.9 [1.1‐266.8] pg/mL respectively), compared to healthy dogs (0.3 [0.2‐74.7]; P < .001 and 0.16 [0.1‐58.1] pg/mL; P < .001), dogs with CKD (0.7 [0.3‐742.5]; P = .04 and 0.37 [0.2‐180.1] pg/mL; P = .03) and CI dogs (1.9 [0.2‐37.0]; P = .03 and 0.8 [0.1‐16.1] pg/mL; P = .02). Fractional excretion of NGAL was significantly higher in dogs with AKI (54.17 [7.93‐155.32] %), than in healthy (0.03 [0.01‐0.21] %; P < .001) and CI dogs (3.05 [0.05‐28.86] %; P = .02).Conclusions and Clinical ImportanceNormalized uTIMP‐2, [uTIMP‐2] × [uIGFBP7]/uCr, and FeNGAL can be valuable renal biomarkers for early diagnosis of AKI in dogs.