Affiliation:
1. State Key Laboratory for Diagnosis and Treatment of Infectious Disease, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases The First Affiliated Hospital, Zhejiang University School of Medicine Hangzhou China
2. Department of Rehabilitation, Shulan (Hangzhou) Hospital Zhejiang Shuren University School of Medicine Hangzhou China
Abstract
Abstract
Aims
Liver damage has caused great illness in human beings. Bifidobacterium catenulatum LI10 has been determined with protective effect against D-galactosamine-induced liver damage. However, due to the sample limitation, the individual difference in its protective effect was not determined. The current study was designed to characterize the gut microbiota of LI10-pretreated rats with lower levels of liver damage.
Methods and Results
A series of experiments and bioinformatic analyses were carried out. Two rat cohorts with different levels of liver damage were determined, that is, Non-Severe and Severe cohorts. Six out of the seven measured liver function variables were lower in the Non-Severe cohort, while four cytokine variables also yielded differences between the two cohorts. The Non-Severe and Severe cohorts were determined with distinct gut microbiota, among which ASV14_Parabacteroides and ASV7_Bacteroides were most associated with Non-Severe and Severe cohorts, respectively. Five phylotypes were determined as structural gatekeepers in the microbiota network of Non-Severe cohort, ASV135_Lachnospiraceae_NK4A136 of which contributed most to the stability of the network.
Conclusions
The relevant findings suggest that some gut bacteria could benefit the protective effect of LI10 on lowering the severity of rat liver damage.
Significance and Impact of the Study
The bacteria benefiting the protective effects of potential probiotics could be further investigated for future clinical application.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
the Independent Task of State Key Laboratory for Diagnosis and Treatment of Infectious Diseases
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology