Ligustrazine improves the compensative effect of Akt survival signaling to protect liver Kupffer cells in trauma‐hemorrhagic shock rats

Author:

Chen Ray‐Jade1,Chen Ming‐Cheng23,Tsai Bruce Chi‐Kang4,Roy Rakesh4,Chang Yi‐Ru1,Wang Tso‐Fu56,Kuo Wei‐Wen78,Kuo Chia‐Hua9,Yao Chun‐Hsu10111213,Li Chi‐Cheng614,Huang Chih‐Yang415161718ORCID

Affiliation:

1. Department of Surgery, School of Medicine, College of Medicine Taipei Medical University Taipei Taiwan

2. Division of Colorectal Surgery, Department of Surgery Taichung Veterans General Hospital Taichung Taiwan

3. Faculty of Medicine National Yang Ming Chiao Tung University Taipei Taiwan

4. Cardiovascular and Mitochondrial Related Disease Research Center Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation Hualien Taiwan

5. Department of Hematology and Oncology Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation Hualien Taiwan

6. School of Medicine Tzu Chi University, Buddhist Tzu Chi Medical Foundation Hualien Taiwan

7. Department of Biological Science and Technology China Medical University Taichung Taiwan

8. Ph.D. Program for Biotechnology Industry China Medical University Taichung Taiwan

9. Department of Sports Sciences University of Taipei Taipei Taiwan

10. Department of Biomedical Imaging and Radiological Science China Medical University Taichung Taiwan

11. School of Chinese Medicine China Medical University Taichung Taiwan

12. Biomaterials Translational Research Center China Medical University Hospital Taichung Taiwan

13. Department of Bioinformatics and Medical Engineering Asia University Taichung Taiwan

14. Center of Stem Cell & Precision Medicine Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation Hualien Taiwan

15. Graduate Institute of Biomedical Sciences China Medical University Taichung Taiwan

16. Department of Medical Research China Medical University Hospital, China Medical University Taichung Taiwan

17. Department of Medical Laboratory Science and Biotechnology Asia University Taichung Taiwan

18. Center of General Education Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology Hualien Taiwan

Abstract

AbstractTrauma‐hemorrhagic shock (THS) is a medical emergency that is encountered by physicians in the emergency department. Chuan Xiong is a traditional Chinese medicine and ligustrazine is a natural compound from it. Ligustrazine improves coronary blood flow and reduces cardiac ischemia in animals through Ca2+ and ATP‐dependent vascular relaxation. It also decreases the platelets' bioactivity and reduces reactive oxygen species formation. We hypothesized that ligustrazine could protect liver by decreasing the inflammation response, protein production, and apoptosis in THS rats. Ligustrazine at doses of 100 and 1000 μg/mL was administrated in Kupffer cells isolated from THS rats. The protein expressions were detected via western blot. The THS showed increased inflammation response proteins, mitochondria‐dependent apoptosis proteins, and had a compensation effect on the Akt pathway. After ligustrazine treatment, the hemorrhagic shock Kupffer cells decreased inflammatory response and mitochondria‐dependent apoptosis and promoted a more compensative effect of the Akt pathway. It suggests ligustrazine reduces inflammation response and mitochondria‐dependent apoptosis induced by THS in liver Kupffer cells and promotes more survival effects by elevating the Akt pathway. These findings demonstrate the beneficial effects of ligustrazine against THS‐induced hepatic injury, and ligustrazine could be a potential medication to treat the liver injury caused by THS.

Funder

China Medical University, Taiwan

Ministry of Science and Technology, Taiwan

Publisher

Wiley

Subject

Molecular Medicine,Biochemistry,Drug Discovery,Pharmacology,Organic Chemistry

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