Affiliation:
1. Department of Nephrology Nantong Hospital Affiliated to Nanjing University of Chinese Medicine, Nantong Hospital of Traditional Chinese Medicine Nantong China
2. Department of Cardiology Nantong Hospital Affiliated to Nanjing University of Chinese Medicine, Nantong Hospital of Traditional Chinese Medicine Nantong China
3. Department of Geriatrics Nantong Hospital Affiliated to Nanjing University of Chinese Medicine, Nantong Hospital of Traditional Chinese Medicine Nantong China
4. Department of Rheumatology Jiangsu Province Hospital of Chinese Medicine Nanjing China
Abstract
AbstractUric acid nephropathy (UAN) is caused by purine metabolism disorders. UAN rat models were established in SD rats. The modeling rats received different doses of hispidulin (10, 20, 50 mg/mL). Febuxostat was applied as the positive drug. Serum creatinine, uric acid (UA), and cystatin‐C (cys‐C), neutrophil gelatinase‐associated lipocalin (NGAL), IL‐1β, IL‐8, TNF‐α, and IL‐6 in rats were detected. HE staining was done to assess kidney injury. UAN rats possessed prominent levels of serum creatinine, UA, cys‐C, and NGAL, which all reduced after hispidulin treatment in a dose‐dependent manner. HE staining determined the improvement of kidney injury after treatment, which was comparable to the efficacy of febuxostat. Hispidulin inhibited the release of IL‐1β, IL‐8, TNF‐α, and IL‐6 in UAN rats. Hispidulin enhanced autophagy in UAN rats, presenting as ascending LC3II/I ratio and downregulated P62. The increasing trend of inflammasome‐related proteins of NLRP3 and Caspase‐1 was changeovered by hispidulin. The activation of NF‐kB signaling was intercepted by hispidulin in UAN rats. Hispidulin can effectively improve renal function injury caused by UAN in rats. The mechanism may be related to the inhibition of inflammatory response induced by autophagy and activation of NF‐κB pathway.
Subject
Molecular Medicine,Biochemistry,Drug Discovery,Pharmacology,Organic Chemistry