Sevoflurane inhibits oral squamous carcinoma progression by modulating the circ_0000857/miR‐145‐5p axis

Abstract

AbstractOral squamous cell carcinoma (OSCC) is a kind of oral malignant tumor with the highest incidence. This study investigated whether sevoflurane (SEV) inhibited OSCC cell progression by regulating circular RNA_0000857 (circ_0000857). OSCC cells were anesthetized with SEV at different concentrations. The expression of circ_0000857 and microRNA‐145‐5p (miR‐145‐5p) were detected by quantitative real‐time polymerase chain reaction (qRT‐PCR). Cell viability was assayed by the Cell Counting Kit‐8 (CCK‐8), and cell migration and invasion were examined by the wound‐healing assay and transwell. Tube formation assay detected angiogenesis. Western blot was used to detect the expression of related proteins. Compared with the control group, SEV inhibited OSCC cell migration, invasion, and angiogenesis. SEV treatment significantly decreased circ_0000857 expression level, but increased miR‐145‐5p expression level in SCC4 and HSC3 cells. MiR‐145‐5p was a target of circ_0000857, and miR‐145‐5p inhibitor reversed the suppressing effects mediated by circ_0000857 silencing on OSCC cell migration, invasion, and angiogenesis. SEV inhibited the level of matrix metalloproteinases 2 (MMP2), MMP9, and vascular endothelial growth factor A (VEGFA) protein by regulating the circ_0000857/miR‐145‐5p axis. In all, SEV regulated the migration, invasion, and angiogenesis of OSCC cells through the circ_0000857/miR‐145‐5p axis, which provided a basis for the potential role of SEV in the treatment of OSCC.