Affiliation:
1. Department of Obstetrics and Gynecology Hebei Medical University Shijiazhuang Hebei China
2. Reproductive Medicine Center The Fourth Hospital of Shijiazhuang Gynecology and Obstetrics Hospital Affiliated to Hebei Medical University Shijiazhuang Hebei China
3. Department of Obstetrics and Gynecology Key Laboratory of Maternal and Fetal Medicine of Hebei Province Shijiazhuang Hebei China
Abstract
AbstractProblemWhether the abnormal development of uterine natural killer (uNK) cells contributes to women with recurrent implantation failure (RIF) remains unclear.Method of studyWe characterized the development of uNK cells and peripheral blood NK cells (pbNK) in the mid‐luteal phase in women with RIF (n = 31) and controls (n = 14) by flow cytometry. Endometrial IL‐15 mRNA expression was studied by quantitative reverse transcription‐PCR. The GSE58144 dataset was used to validate the correlation results.ResultsWe found decreased proportions of stage 4 CD56+CD16‐CD94+ uNK cells (median: 9.56% vs. 17.78%, P .014) and increased proportions of stage 6 CD56+CD16+CD57+ uNK cells (median: 1.54% vs. 0.74%, P = .020) in the mid‐luteal endometrium of women with RIF compared to fertile women. We also found that there was no quantitative correlation between uNK cells and the corresponding pbNK cell subpopulations (P > .05). In addition, IL‐15 mRNA levels in the mid‐luteal endometrium were positively correlated with the proportion of CD56+ uNK cells (r = .392, P = .008), especially with stage 4 uNK cell populations (r = .408, P = .005).ConclusionsWe showed that the proportion of stage 4 uNK cells decreased in the RIF group compared to controls, and the decrease in stage 4 uNK cells correlated positively with low IL‐15 mRNA expression. We suggest that the reduced stage 4 uNK cells in women with RIF are associated with IL‐15 deficiency.
Funder
Natural Science Foundation of Hebei Province
Hebei Provincial Department of Human Resources and Social Security
Subject
Obstetrics and Gynecology,Reproductive Medicine,Immunology,Immunology and Allergy,Obstetrics and Gynecology,Immunology